Toxic Effects of Methanolic Extracts of Plants Leaves on the Mortality and Enzymatic Parameters of Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) Juveniles

Diligent Efiong Oboho *

Department of Animal and Environmental Biology, University of Uyo, Nigeria.

Rosemary Anietie Essien

Department of Crop Science, Akwa Ibom State University, Obio Akpa Campus, Nigeria.

Emem Okon Mbong

Department of Environmental Biology, Heritage Polytechnic, Ikot Udota Eket, Nigeria.

Henry D. Akpan

Department of Biochemistry, University of Uyo, Akwa Ibom State, Nigeria.

Akwaowo Uduehe Nelson

Department of Animal Science, Federal Polytechnic Ugep, Cross River State, Nigeria.

Benjamin Uzonna Ononye

Department of Zoology, Nnamdi Azikiwe University, Awka Anambra State, Nigeria.

Udo Inyang John

Department of Biological Sciences, Akwa Ibom State Polytchnic, Ikot Osurua, Nigeria.

Joseph Effiong Eyo

Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

This study was conducted in the laboratory to evaluate the effect of leaves extract of three botanicals on the biochemical parameters of insect pest of stored beans, Callosobruchus maculatus. The results of the qualitative phytochemicals revealed the presence of different metabolites such as tannins, saponins, cardiac glycosides, phenolic compounds, flavonoids, alkaloids etc. The toxic effect of methanolic leaves extracts of Lasianthera africana, Hippocratea africana and Uvaria chamae on the mortality and biochemical parameters after treatment against C. maculatus was evaluated. The result showed that treatment of the insects with these extracts significantly increased mortality in C. maculatus. There was a significant inhibition of the enzymatic activities of the digestive enzymes (such as amylase and invertase) in the treated larvae. The transaminase enzymes (AST and ALT) were found to be reduced in the insect after treatment (33.2 and 42.6) while the result of the phosphatase (ACP and ALP) enzyme activity  showed a potent inhibitory effect of the leaves extract, which was more pronounced in ALP (75.8%) than ACP (31.8%).

Keywords: Callosobruchus maculatus, Lasianthera africana, Hippocratea africana, Uvaria chamae, enzymatic, toxicity


How to Cite

Oboho , D. E., Essien , R. A., Mbong , E. O., Akpan , H. D., Nelson , A. U., Ononye , B. U., John , U. I., & Eyo , J. E. (2023). Toxic Effects of Methanolic Extracts of Plants Leaves on the Mortality and Enzymatic Parameters of Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) Juveniles . Asian Journal of Research in Zoology, 6(2), 1–10. https://doi.org/10.9734/ajriz/2023/v6i2106

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