Effect of Foeniculum vulgare Mingled Diet upon Growth, Reproduction and Spawning Performance of Guppy Fish

Mamoona Kiran Zareen *

University of Lahore, Pakistan.

Muhammad Sultan Haider

University of Lahore, Pakistan.

Nasim Akhtar

University of Lahore, Pakistan.

Sadia Fatima

University of Lahore, Pakistan.

Adeela Hassan

University of Lahore, Pakistan.

*Author to whom correspondence should be addressed.


Naturally breeding fish cannot meet the demand of humans. So induced breeding is essential to fulfill this demand. Induced breeding ensures a timely supply of fish seeds having desired characteristics and different techniques can be used for this purpose. In this perspective synthetic hormones, extracts of pituitary hormones, Gonadotropin-releasing hormones, and sometimes Human chronic gonadotropin are used as a spawning inducer for fishes. Plant extracts can also be used as it is a new technique which could be used in aquaculture. The basic aim of our study was to enhance the growth as well as to check the effect of the traditional herb Foeniculum vulgare on the reproductive performance of Poecilia reticulata.

Methodology: Samples were collected from a local fish shop in Sargodha. Ethanolic extract of Foeniculum vulgare seeds was prepared and mixed with different commercial feed. This feed was given to fish for 90 days to test the effect of Foeniculum vulgare seed extract on growth and reproduction performance. Water quality parameters were also maintained and recorded daily. Fecundity and Gonad somatic index were measured after dissection by removing the ovaries. Fecundity was calculated by counting the number of larvae and eggs seen with the naked eye. The hatching rate was measured by counting the number of hatching in each spawning.

Results: According to the growth results, different levels of fennel seed extract has a significant effect on weight gain, length gain and survival rates of the fish in different groups. Statistical analysis cleared that T2 showed the highest weight and length gain. The feed Conversion Ratio decreased significantly. Results of reproduction parameters showed that the highest absolute fecundity (9.4 ± 0.19), GSI (8.69 ± 0.2) and spawning have increased in T2 followed by T1, T3, and control respectively. The observation of present research shows that Foeniculum vulgare seed extract can act as an estrogenic compound and growth promoter in commercial aquaculture.

Keywords: Foeniculum vulgare, fecundity, spawning, gas chromatography mass spectroscopy, reproductive performance

How to Cite

Zareen , M. K., Haider , M. S., Akhtar , N., Fatima , S., & Hassan, A. (2023). Effect of Foeniculum vulgare Mingled Diet upon Growth, Reproduction and Spawning Performance of Guppy Fish. Asian Journal of Research in Zoology, 6(4), 88–99. https://doi.org/10.9734/ajriz/2023/v6i4126


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Nasser AKV, MMM Rajkumar U. Ornamental fish-prospects for culture. Souvenir issued on the Occasion of the inauguration of Visakhapatnam RC of CMFR. 2001;24-26.

Rodrìguez RDR. Ornamental fish: Economic, ecologic and health implications of the trade. JAINA Boletin Informativo. 2006;16(1):6-27.

Ojanguren. Monographic study of the scorpions of the Argentine Republic. Iberian Journal of Arachnology. 2005; 11:75-241.

Egset CK, Bolstad GH, Rosenqvist G, Endler JA, Pelabon Cz. Geographical variation in allometry in the guppy (Poecilia reticulata). Journal of Evolutionary Biology. 2011;24(12):2631-2638.

Hildebrand SF. Suggestions for a broader application of Gambusia for the purpose of mosquito control in the South. Public Health Rep. 1921;36:1460–1461. DOI: 10.2307/4576031

Coad BW. Review of the livebearer fishes of Iran (Family: Poeciliidae). Iranian Journal of Ichthyology. 2017;4(4):305-330.

Sudha C, Gokula V. Induced spawning of rosy barb Puntius conchonius by Natrum muriaticum. IJAPBC. 2015;4(4):801-804

Ojima D, Iwata M. Central administration of growth hormone-releasing hormone triggers downstream movement and schooling dbehavior of chum salmon (Oncorhynchus keta) fry in an artificial stream. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2009;152(3):293-298.

Ghosh S, Sinha A, Sahu C. Effect of probiotic on reproductive 18-526.methods by GC/MS. African Journal of Traditional, Complementary and Alternative Medicines. 2007;11(2):277-279.

Yanong RP, Martinez C, Watson CA. Use of Ovaprim in ornamental fish aquaculture. EDIS. 2010;2010(2).

Hunter JR, Macewicz BJ, Kjesbu OS, Hunter JR, Witthames P. Improving the accuracy and precision of reproductive information used in fisheries, Report of the Working Group on Modern Approaches to Assess Maturity and Fecundity of Warm and Coldwater Fish and Squids, BergenFisken og havet, 12, Institute for Marine Research. 2003;57-68.

Chavan Vinay, Muley Deepak. Effect of heavy metals on the liver and gill of fish Cirrhinus mrigala. Int. J. Curr. Microbiol. Appl. Sci. 2014;3:277-288.

Nazari A, Roozbehani S. Influence of fennel Foeniculum vulgare extract on fertility, growth rate and histology of gonads on guppy Poecilia reticulata Turkish Journal of Fisheries and Aquatic Sciences. 2015;15(3):457-463.

Alam Md. Morshedul. The essence of antioxidants in aging science: NRF2, a true fact. 2019;5:1-5.

Krumholz LA. Reproduction in the western mosquitofish, Gambusia affinis affinis (Baird & Girard), and its use in mosquito control. Ecological Monographs. 1948; 18(1):1-43.

Salehi H. Benefit-cost analysis for fingerling production of kutum Rutilus frisii kutum (Kamensky, 1901) in 2005 in Iran. Aquac. Asia Mag. 2008;13(3):35.

Frankic T, Voljc M, Salobir J, Rezar V. Use of herbs and spices and their extracts in animal nutrition. Acta Agric Slov. 2009;94(2):95-102.

Yeganeh S. Department of fisheries, faculty of animal sciences and fisheries, Sari Agricultural Sciences and Natural Resources University, Km 9 Darya Boulevard, PO Box 578, Sari, Iran.

Yilmaz S, Ergün S, Turk N. Effects of cumin-supplemented diets on growth and disease (Streptococcus iniae) resistance of tilapia (Oreochromis mossambicus). The Israeli Journal of Aquaculture – Bamidgeh. 2012;64:5.

Yılmaz S, Sebahattin E, Celik E. Effects of herbal supplements on growth performance of sea bass (Dicentrarchus labrax): Change in body composition and some blood parameters. Energy (kJ/g). 2010;1(3):217-222.

Dada AA. Effect of Garcinia kola seed meal on egg quality of the North African catfish (Clarias gariepinus) (Burchell, 1822) broodstock. African Journal of Food, Agriculture, Nutrition and Development. 2012;12(5):6447-6459.

Sadeghpour A, Alizadehasl A, Kyavar M, Sadeghi T, Moludi J, Gholizadeh F, Totonchi Z, Ghadrdoost B. Impact of vitamin C supplementation on post-cardiac surgery ICU and hospital length of stay. Anesth Pain Med. 2015;5(1):e25337. DOI: 10.5812/aapm.25337. PMID: 25789244; PMCID: PMC4350190.

Chatterjee S, Biondi-Zoccai G, Abbate A, D'Ascenzo F, Castagno D, Van Tassell B, Mukherjee D, Lichstein E. Benefits of β blockers in patients with heart failure and reduced ejection fraction: network meta-analysis. BMJ. 2013;346:f55. DOI: 10.1136/bmj.f55. Erratum in: BMJ. 2013;346:f596. PMID: 23325883; PMCID: PMC3546627.

Nielsen L, Baatrup E. Quantitative studies on the effects of environmental estrogens on the testis of the guppy, Poecilia reticulata. Aquat Toxicol. 2006;80(2):140-8. DOI: 10.1016/j.aquatox.2006.08.004. Epub 2006 Aug 22. PMID: 16997393.

Yilmaz E, Genc MA, Cek S, Mazlum Y, Genc E. Effects of orally administered Ferula coskunii (Apiaceae) on growth, body composition and histology of common carp, Cyprinus carpio. Journal of Animal and Veterinary Advances. 2006;5(12):1236-1238.

Ananda K, Sridhar KR. Diversity of endophytic fungi in the roots of mangrove species on the west coast of India. Can J Microbiol. 2002;48(10):871-8. DOI: 10.1139/w02-080. PMID: 12489776.

Sudha, Gokula Murthy, Reddy,Shravan. Psychogenic non-epileptic seizures: A case report; 2018. Available:10.13140/RG.2.2.20321.81766

Hildebrand SF. Suggestions for a broader application of Gambusia for the purpose of mosquito control in the South. Public Health Rep. 1921;36:1460–1461. DOI: 10.2307/4576031

Haghighi Mohammad, Dorosti Ali, Rahnama Afshin, Hoseinpour Ali. Evaluation of factors affecting customer loyalty in the restaurant industry. African Journal of Business Management. 2012; 6. Available:10.5897/AJBM11.2765

Tahar Hammouda, Shantanu Keshav. Melting in the mantle in the presence of carbon: Review of experiments and discussion on the origin of carbonatites, Chemical Geology. 2015;418:171-188. ISSN 0009-2541