Microbial Assessment of Fresh and Frozen (For Four Days) Marine Najil Fish (Plectropomus pessuliferus)

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Khansaa Bdr Aldien Osman Ahmed
Samia Hamid Ahmed Hamid
Abdalla N. Elawad


The present study was conducted at Directorate General of Preventing Medicine and PHC, Epidemiology Dept, Ports and Quarantine Health Unit, Port Sudan, endeavors for Microbial evaluation (bacteriological load) of marine Najil fish (Plectropomus pessuliferus). And to determine the occurrence of some contaminated bacteria. 20 samples of Najil fish (P. Pessuliferus) were collected from Port Sudan fish market (in sterile plastic bags) and pre chilled with ice in thermostatic container. All the samples were tested immediately, and instantly froze for four days. Then the studied samples were tested microbiologically and results showed:For fresh fish total bacterial count (TBC) was (5.68×105) CFU/gm, and for frozen samples was (4.38×105) CFU/gm, respectively.The study concluded that, Najil fish (P. Pessuliferus) showed the highest level of contamination between fresh fish. While in frozen fish freeze seem have the higher effect (less number). And the results showed highly significant differences between fresh and frozen fish.The samples were also tested for contaminant bacteria and the result indicated the presence of Staphylococcus and E. coli, while the salmonella and vibrio were absent at each of it.

Microbial evaluation, frozen fishes, Najil (Plectropomus pessuliferus) fish.

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How to Cite
Ahmed, K. B. A. O., Hamid, S. H. A., & Elawad, A. N. (2020). Microbial Assessment of Fresh and Frozen (For Four Days) Marine Najil Fish (Plectropomus pessuliferus). Asian Journal of Research in Zoology, 3(4), 1-12. https://doi.org/10.9734/ajriz/2020/v3i430095
Original Research Article


FAO. Food and Agriculture Organization. The state of World Fisheries and Aquaculture. Rome. 2014;223.

Adebayo-Tayo AC, Odu NN, Michael MU, Okonko IO. Multi-Drug Resistant (MDR) organisms isolated from Sea-foods in Uyo, South-Southern Nigeria. Nature and Science. 2012;10(3):61-70.

Begum K. Comparative microbiological assessment of export oriented fishes and locally marketed fishes.MSC. Dhaka University Institutional Repository. 2014;1-85.

Begum M, Abu Ahmed A, Das M, Parveen S. A comparative microbiological assessment of five types of selected fishes collected from two different markets. Advances in Biological Research. 2010;4(5):259-265.

SSMO. (Sudanese Standard Metrology Organization, SDS 357; 2008.

Ahmed S, Bdreldien K, Abdella R. Survey of contaminant bacteria on Oreochromis niloticus and lates niloticus at Elmourda fish market. Direct Research Journal of Agriculture and Food Science (DRJAFS). 2014;2(7):98-101.

Barrow GL, Feltham RK. Cowan steel manual of Identification of medical microbiology bacteria 3d edition Cambridge University press. 1993;315.

Jorge RJ, Redondo PN, Dosta MD. Bacterial load comparison of marine fish collected and commercially obtained for human consumption in western region of Yucatan Peninsula, Mexico. International Journal of Aquatic Science. 2016;7(1):6-12.

ICMSF (International Commission on Microbiological Specifications for foods). 1998. Micro-organisms in foods: Microbial ecology of food commodities. London: Blackie Academic and Professional.

Adebayo-Tayo BC, Odu NN, Anyamele, L. M., Igwiloh, N. J and Okonko, I. O. 2012. Microbial quality of frozen fish sold in Uyo Metropolis. Nature and Science; 10 (3):71-77.

Noor R, Acharjee M, Ahmed T, Das KK, Paul L, Munshi SK, Urmi NJ, Rahman F, Alam MZ. Microbiological study of major sea fish available in local markets of Dhaka City, Bangladesh. Journal of Microbiology, Biotechnology and Food Sciences. 2013;2(4):2420- 2430.

Oramadike CE, Ibrahim AO, Kolade OY. Biochemical and microbiological quality of frozen fishes available in some supermarkets in Lagos State, Nigeria. Journal of Life and Physical Sciences. 2010;3(2):48–51.

Nilla SS, Khan NA, Khan MM, Ahsan DA, Mustafa MG. Bacteriological quality of marketed Mola Fish, AmblypharyngodonMolafrom Dhaka Metropolis. Bangladesh J. Zoo. 2012;40(1):77-88.

FAO. Food and Agriculture Organization. Manual of good quality control for microbiological analysis. Food and Agriculture Organization of the United Nation, Roma, Italy. 1979;144. ISBN-13:9789251008492.

Losito F, Bottini G, de Ascentis A, Priolisi F, Mari A, Tarsitani G, Antonini G. Qualitative and quantitative validation of the microbiological survey method for Listeria spp., Salmonella spp., Enterobacteraceae and Staphylococcus aureus in food samples. A M.J. Food Technology. 2012;7:340-351.