Main Article Content
Enzymes form integral part and play decisive role in biological Systems and metabolic network. The present studies delve on the role of enzymes viz., invertase, amylase, glucose oxidase and catalase in nectar-honey transformation during May 2019 to April 2020. Data on enzymatic activity in all the five stages related to honey formation from nectar i.e., floral nectar (fn), honey crop of foragers (hf), honey crop of house bees (hh), unsealed honey cells (uh) and sealed honey cells (sh) was evaluated. Invertase in fn and sh cells was found minimum of 0.00 ml and maximum of 42.40 ml, respectively. Similarly, amylase in the fn was least (0.00 ml) and highest in sh (16.01 ml) and catalase was observed to be 0.001 ml in floral nector and highest in sealed honey cells i.e., 16.01 ml and 4.96 ml,respectively. Accordingly, glucose oxidase in fn and sh cells was minimum of 0.00 ml and maximum of 6.68 ml respectively. Correspondingly, catalase in the fn was least (0.00 ml) and highest in sh (4.96 ml). The analysis of variance of invertase and amylase was significant at 1 % level (p<0.01), whereas glucose oxidase and catalase Were statistically non-significant at 1 % level (p<0.01). Results have confirmed that the sources of all enzymes are instigated from hypopharyngeal, post-cerebral, thoracic, labial and mandibular glands of foragers and house bees which are accountable for honey formation. Further, each enzyme is substrates specific which are discussed in ensuing paper.
Anita H, Atmowidjojo, Eric H. Erickson. Neem, a potentially valuable nectar and pollen resource for beekeeping in Indonesia. Indian Bee J. 1998;60(2):101- 105.
Crane E. Beekeeping in mountain life-support systems. In: Honeybees in Mountain Agriculture, Verma, L.R. (Ed.) Oxford and IBH Publ. Co. New Delhi. 1992;17-27.
Balasubramanyam MV, Reddy CC. Physical characteristics of apiary and wild honey from plains, hills and Western Ghats of Karnataka. Indian Bee J. 2003; 65(3-4):111-117.
Joshi SR, Pechhacker H, William A, Vonder Ohe W. Physico-chemical characteristics of A. dorsata, A. dorsata and A. mellifera honey of Chitwan district, Central Nepal. Apidologie. 2000;31:367-375.
Shripad N. Agashe, Rangaswamy BE. Chemical characterization of Apis cerana F and Apis dorsata F. honey from Dakshina Kannada, Karnataka (India). Indian Bee J. 2001;63 (3-4):15-20.
Balasubramanyam MV, Reddy CC. Mineral variations of honey of indigenous honeybee species from Western Ghats of Karnataka. J. Pharm. Res. Clini. Practice. 2011;1(2): 36-42.
BIS. Indian Standard Specification for Extracted Honey, New Delhi. 1997;10-13.
Wakhle DM, Desai DB. Enzymes in Indian Honeys. II Int. Conf. on Apicult. in Trop. Climates, New Delhi, in Proceedings. 1983;703-710.
Joint Association of Analytical Chemists. 2001;594-606.
Balasubramanyam MV. Factors influencing the ripening of honey. Ph.D Thesis, Bangalore University, Bangalore. 2006;43-48.
Bradbear N. Beekeeping in rural development. IBRA, London. 1990;1-30.
Balasubramanyam MV. Quantitative chemical variations in ripening of honey of indigenous hive bee Apis dorsata. Intern. J. of App. Bio. and Pharm. Tech. 2011; 2(3):391-397.
Balasubramanyam MV. Chemical characteristics of Multifloral wild and apiary honey from Western Ghats of Karnataka. Intern. J. of Life Sciences. 2011;6(3):467–469.
Wakhle DM. Estimation of antibacterial activity f some Indian honeys. Indian Bee. J. 1991;53:89-90.
White JW. The role of HMF and diastase assays in honey quality evaluation. Bee Wld. 1994;75:104-113.